6.8.1 HPLC chromatographic system/condition 高效液相色谱系统/条件
Chromatographic column: A stainless steel column packed with a irregular, porous silica gel to which benzene sulphonic acid groups have been chemically bonded (10 μm) or equivalent.
色谱柱：填充不规则多孔硅胶，化学键合苯磺酸基团的不锈钢柱(10 μm)或类似柱。
Size 尺寸：4.6 mm × 25 cm
Wavelength 波长：232 nm
Injection volume 进样量：20 μL
Flow rate 流速：1 mL/min
Mobile Phase: 1.7% w/v solution of ammonium dihydrogen orthophosphate adjusted to pH 3.0 with orthophosphoric acid.
流动相：1.7%w/v磷酸二氢铵溶液，用磷酸调节pH至3.0。
供应 盐酸二甲双胍分析用色谱柱