DIG 3*末端寡核苷酸标记试剂盒
DIG Oligonucleotide 3'-End Labeling Kit, 2nd generation:Formulation containing recombinant Terminal Transferase Kit for 25 labeling reactions of 100 pmol of oligonucleotides
产品号
产品规格
厂商
价格
03353575910
(3353575)
1 kit (25 labeling reactions)
Roche
6325
Application3'-end labeling of oligonucleotides from 14 to 100 nucleotides in length with DIG-11-ddUTP and recombinant Terminal Transferase. DIG-labeled oligonucleotides are used as hybridization probes in all types of membrane-blot applications and in situ hybridization.BenefitsFast hybridization kinetics, due to the small size of oligonucleotides Single-stranded probes, no renaturation during hybridization Sequence can be designed according to the experiment Specially suited for in situ hybridization; due to their small size, the oligonucleotides readily diffuse into fixed tissues and cells Contents1. Reaction Buffer 5x, 100 µl 2. CoCl2 Solution, 100 µl, 25 mM 3. DIG-ddUTP Solution, 25 µl, 1 mM 4. Recombinant Terminal Transferase, 25 µl, [400 U/µl] 5. Control Oligonucleotide, unlabeled, 25 µl, [20 pmol/µl] 6. Oligonucleotide, DIG-ddUTP labeled, 100 µl, [2.5 pmol/µl] 7. Control DNA, 20 µl, [2.5 pmol/µl] pUC 18 DNA, supercoiled 8. Glycogen Solution, 50 µl, [20 mg/ml] 9. DNA Dilution Buffer, 1 ml, [50 µg/ml] fish sperm DNA PrincipleOne DIG-ddUTP molecule is added to the 3'-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction
DIG 3*末端寡核苷酸标记试剂盒