LAS ELISA Kit直链烷基苯磺酸盐ELISA试剂盒
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LAS ELISA Kit直链烷基苯磺酸盐ELISA试剂盒
详细信息 LAS ELISA Kit直链烷基苯磺酸盐ELISA试剂盒 Anionic Surfactant LAS ELISA Kit • The monoclonal antibody binds exclusively with LAS and does not cross-react with other surfactants or compounds of similar structure. • The detection range is between 0.02mg/L and 1mg/L, sensitive enough to render harmful solvent extraction and concentration unnecessary. • A simple filtration through glass filter is the only pretreatment before measurement. • With ease of handling, the total time for measurement is only 2.5 hours. • The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost. LAS LAS is an abbreviation for Linear Alkylbenzene Sulfonate, the most widely used synthetic detergent and the major component among methylene blue active substances (MBAS). LAS has been pointed out to generate fish toxicity through bioaccumulation. LAS Standard Curve The sample containing LAS within the dynamic range (0.02mg/L-1mg/L) can be directly applied to assay after filtration. Coefficient of variation (CV) is generally under 10% throughout the dynamic range. • A concentration is necessary for a sample with LAS content below the range for quantification. Comparison with Conventional Methods ELISA exhibits high correlation with MBAS and HPLC. The value from ELISA is almost equivalent to that of HPLC, which measures only LAS, and is smaller than that of MBAS detecting other anionic surfactants as well. Cross-Reactivity Pattern Compound % reactivity Anionic surfactants Linear alkylbenzene sulfonates(LAS) C12 100 C11 87 C10 101 C9 114 C8 75 Sodium dodecyl sulfate(SDS) 1.0 Sodium myristate 0.3 Sodium laurate 0.1 Sodium palmitate <0.1 Sodium stearate <0.1 Nonionic surfactants Nonylphenol ethoxylate(EO=10) <0.1 Polyoxyethylene(EO=20) sorbitan monolaurate <0.1 Disodium lauryl surlfosuccinate <0.1 Benzenesulfonic acid <0.1 Phenol <0.1 Toluene <0.1 Xylene <0.1The monoclonal antibody has a high specificity to LAS with various alkyl chain length (n=8-12) and doesn't cross-react with other surfactants or compounds of similar structure. Kit Format Kit Format Comment Microplate 1 plate (96 wells) & reagents Needs a microplate reader(450nm) For multiple and simultaneous measurement如有更多其它试剂产品需求与查询,请及时接洽:info@express-cn.com 或在我公司查询主页客户留言处(http://www.express-cn.com/expressgb/lyb.htm )留下您的查询内容,我们即会在当天为您作出回复。伊普瑞斯科技有限公司 info@express-cn.com 在线客服:QQ:1069267096 MSN:liyuqiu1983@hotmail.com cindylee-1981@hotmail.com 联系人:杨先生 总机:010-60206266 传真:010-60206773 www.express-cn.com |